If you plug a USB mouse into his Mac, does it work? Does the drive mount on another machine?
USB ports are managed by an extension file - /System/Library/Extensions/IOUSBMassStorageClass.kext
If it has been deleted, then the USB ports will be disabled.
There is a Configuration Profile that can disable external Hard Drives.

There's also a setting in the restriction payload for config profile that can prevent ejecting external drives, it's in the same area as the restriction fo "go to folder", "connect to sever" etc.
Could be worth checking in there as well.
Possible?
[https://jamfnation.jamfsoftware.com/viewProductFile.html?fid=455](link URL)
/Library/Application Support/JAMF/DisabledExtensions/
Yes i tried read-only USB but one time works me and after not blocking the Pendrive
This is Deprecated. What is the new way to do this?
This is Deprecated. What is the new way to do this?
Have you guys got any insights on this to disable USB??